Comparative Analysis of the Application of Polystyrene Microspheres and Polystyrene Carboxyl Microspheres in Biotechnology – Focusing on Nucleic Acid Removal.
(LNJNbio Polystyrene Microspheres)
In the area of contemporary biotechnology, microsphere materials are extensively made use of in the removal and purification of DNA and RNA as a result of their high specific surface area, good chemical stability and functionalized surface buildings. Amongst them, polystyrene (PS) microspheres and their obtained polystyrene carboxyl (CPS) microspheres are one of the two most extensively studied and applied products. This article is given with technical assistance and information evaluation by Shanghai Lingjun Biotechnology Co., Ltd., aiming to methodically compare the performance distinctions of these 2 kinds of products in the procedure of nucleic acid extraction, covering crucial signs such as their physicochemical residential or commercial properties, surface adjustment capacity, binding efficiency and recovery rate, and highlight their suitable situations with speculative information.
Polystyrene microspheres are homogeneous polymer bits polymerized from styrene monomers with excellent thermal stability and mechanical strength. Its surface is a non-polar structure and generally does not have active functional teams. As a result, when it is straight utilized for nucleic acid binding, it needs to depend on electrostatic adsorption or hydrophobic activity for molecular fixation. Polystyrene carboxyl microspheres introduce carboxyl useful groups (– COOH) on the basis of PS microspheres, making their surface efficient in further chemical coupling. These carboxyl teams can be covalently bonded to nucleic acid probes, proteins or other ligands with amino teams via activation systems such as EDC/NHS, therefore attaining more steady molecular addiction. Therefore, from an architectural point of view, CPS microspheres have more advantages in functionalization potential.
Nucleic acid removal normally includes actions such as cell lysis, nucleic acid release, nucleic acid binding to strong stage providers, cleaning to eliminate pollutants and eluting target nucleic acids. In this system, microspheres play a core duty as solid phase providers. PS microspheres generally depend on electrostatic adsorption and hydrogen bonding to bind nucleic acids, and their binding efficiency is about 60 ~ 70%, but the elution efficiency is low, just 40 ~ 50%. In contrast, CPS microspheres can not only utilize electrostatic effects however likewise achieve even more solid fixation with covalent bonding, decreasing the loss of nucleic acids throughout the cleaning process. Its binding effectiveness can reach 85 ~ 95%, and the elution efficiency is likewise increased to 70 ~ 80%. Additionally, CPS microspheres are additionally considerably much better than PS microspheres in terms of anti-interference ability and reusability.
In order to verify the efficiency differences in between the two microspheres in actual procedure, Shanghai Lingjun Biotechnology Co., Ltd. performed RNA removal experiments. The experimental samples were originated from HEK293 cells. After pretreatment with conventional Tris-HCl barrier and proteinase K, 5 mg/mL PS and CPS microspheres were used for extraction. The outcomes revealed that the average RNA return extracted by PS microspheres was 85 ng/ Ī¼L, the A260/A280 proportion was 1.82, and the RIN worth was 7.2, while the RNA yield of CPS microspheres was boosted to 132 ng/ Ī¼L, the A260/A280 ratio was close to the ideal value of 1.91, and the RIN value reached 8.1. Although the procedure time of CPS microspheres is somewhat longer (28 minutes vs. 25 mins) and the price is greater (28 yuan vs. 18 yuan/time), its removal quality is considerably boosted, and it is more suitable for high-sensitivity discovery, such as qPCR and RNA-seq.
( SEM of LNJNbio Polystyrene Microspheres)
From the viewpoint of application situations, PS microspheres are suitable for large screening jobs and preliminary enrichment with reduced demands for binding specificity as a result of their affordable and straightforward operation. However, their nucleic acid binding ability is weak and easily affected by salt ion focus, making them unsuitable for lasting storage space or repeated use. In contrast, CPS microspheres appropriate for trace example removal because of their abundant surface practical groups, which assist in more functionalization and can be made use of to create magnetic grain discovery kits and automated nucleic acid extraction systems. Although its preparation procedure is reasonably intricate and the expense is relatively high, it reveals stronger versatility in scientific research and professional applications with strict demands on nucleic acid extraction effectiveness and purity.
With the rapid development of molecular medical diagnosis, gene modifying, fluid biopsy and other areas, higher demands are placed on the efficiency, pureness and automation of nucleic acid extraction. Polystyrene carboxyl microspheres are progressively replacing standard PS microspheres due to their outstanding binding performance and functionalizable characteristics, ending up being the core option of a brand-new generation of nucleic acid removal products. Shanghai Lingjun Biotechnology Co., Ltd. is also continuously optimizing the particle size distribution, surface area thickness and functionalization effectiveness of CPS microspheres and establishing matching magnetic composite microsphere products to fulfill the demands of medical diagnosis, scientific study institutions and industrial consumers for high-quality nucleic acid extraction services.
Distributor
Our products are widely used in many fields, such as medical testing, genetic testing, university research, genetic breeding and more. We not only provide products but can also undertake OEM, ODM, and other needs. If you need kit for dna extraction, please feel free to contact usĀ atĀ sales01@lingjunbio.com.
All articles and pictures are from the Internet. If there are any copyright issues, please contact us in time to delete.
Inquiry us